Overview of Products & Services

Affomix is built on the premise that simultaneous tracking and quantitation of the proteome will dramatically increase our understanding of cellular phenomena.  Achievement of such protein characterization ideally requires a vast matched library of specific mAbs and quantitation technology with adequate sensitivity and dynamic range. Armed with such mAb libraries, we can better probe the behavior of signal transduction pathways or of proteins from specific classes of genes, e.g. oncology panels. Additionally, it is desirable to characterize post-translational modifications of proteins because these modifications control protein activity and behavior, which in turn influence regulatory pathways. 

Unmet Need: Current technologies limit the achievement of these goals as the methods used for selecting Abs are throughput-limited, large sets of mAbs with matched properties are unavailable, methods for quantitation of proteins in multiplexed arrays are limiting and technologies available for measurement of post-translationally modified proteins is neither high throughput nor adequately sensitive. 

Solution:  Affomix has created a suite of technologies designed to accomplish these goals.

Current Offering

Y2HEXPRESSTM

Yeast two hybrid (Y2H) is a process for selecting mAbs against non-modified proteins. Standard Y2H methodology is well established and carries the advantages of selection without the need for production of antigen as well as specific epitope targeting. However, the process has been limited by throughput and specificity issues. 

Affomix has developed Y2HEXPRESSTM, a proprietary automated approach that dramatically increases the speed and specificity at which mAb selection and production can be carried out. 

The Y2HEXPRESSTM breakthrough advantages:

  • Greater than 50X increase in throughput over standard Y2H

  • Greater than 5X increase in selection specificity

  • mAb selection complete in 6-8 weeks

  • In-house services with the ability to process 150 targets per week and scalable

Y2HEXPRESSTM is provided as a service, whereby all our clients have to do is identify the target proteins. Affomix will do everything else – from picking the epitopes (using a proprietary algorithm) to bait construction and mAb selection – all within 6-8 weeks.

Please contact us at sales@affomix.com to learn more and to begin a service today.

Future Offerings

Affomix has leveraged its proprietary technology to enable advances in proteomics research. These advances are centered on:

  • Phage ESCapeTM- Utilizing advances in fluidics technologies to detect and quantitate post-translationally modified proteins versus their unmodified counterparts

  • Solution analysis of mAb-antigen interactions - Allowing protein-protein interaction reactions to be carried out in solution and captured on chips or beads for semi-quantitative measurement

  • Quantitation of mAb-antigen interactions - Interrogating mAb-antigen interactions on a substrate, for quantitative measurement